Publicerade inlägg i: Övrigt i New York City | Posted: |
Ca2+ mobilization is the result of activation of GPCRs binding to specific Gα subunits Gq and Gi. Gq-coupled receptors activate the classical phospholipase C pathway, leading to an increase in inositol triphosphate (IP3) level. IP3 binds to IP3-sensitive calcium channels on the endoplasmic reticulum resulting in the release of calcium from the endoplasmic reticulum into the cytoplasm. Changes in intracellular calcium that reflect GPCR activity can be measured conveniently and accurately using calcium-sensitive dyes.